Direct versus indirect designs for cDNA microarray experiments
نویسندگان
چکیده
We calculate the variances of two classes of estimates of differential gene expression based on log ratios of fluorescence intensities from cDNA microarray experiments: direct estimates, using measurements from the same slide, and indirect estimates, using measurements from different slides. These variances are compared and numerical estimates are obtained from a small experiment involving 4 slides. Some qualitative and quantitative conclusions are drawn which have potential relevance to the design of cDNA microarray experiments. AMS Subject Classification, Primary: 92B15; Secondary: 62H99, 62J10
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